In agarose gel electrophoresis, a sample referred to as “marker” is typically included. What is a marker?
The term refers to a DNA molecular weight marker (DNA ladder), which is a series of known size DNA fragments used as a reference to judge the sizes of bands that will appear after electrophoresis. The term “ladder” comes from the series of step-like bands formed by these DNA fragments on the gel, with each band representing a different size of DNA molecule.
The use of markers in gel electrophoresis has several key aspects:
Molecular Weight Standard: Markers contain multiple known-size fragments, which can be DNA or proteins. By comparing the migration distance of the samples with that of the marker, the relative sizes of the sample molecules can be estimated.
Quality Control: Using a marker can help confirm the success of the electrophoresis experiment. If the marker bands appear normal and consistent in size, it indicates that the experimental conditions are appropriate and the samples have been processed correctly.
Localization and Verification: When analyzing the results, markers can also help locate specific target molecules. If you know the size of a target molecule, the marker can assist in confirming whether it is present in the expected position.
Different Types of Markers: Depending on the experimental needs, different types of markers may be used. For example, DNA ladders are commonly used for DNA electrophoresis, while protein molecular weight standards are used for protein electrophoresis.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) offers different models of horizontal electrophoresis tanks for agarose gel electrophoresis. Different gel casting device for casting small gels to big gels, like gel size 60mmx60mm, 250mmx250mm.
Selection Guide for Horizontal Immersed Gel Electrophoresis Unit
|
Model |
Gel size(W*L) mm |
Buffer Volume ml |
Comb Rows |
No.of Samples |
| DYCP-31BN | 60*60 | 120 | 1~2 | 8~11 |
| DYCP-31CN | 70*100 | 260 | 1~4 | 8~15 |
| DYCP-31DN | 120*120, 60*120, 120*60, 60*60 | 650 | 1~4 | 2~72 |
| DYCP-31E | 160*200, 160*150 | 1000 | 1~6 | 17~204 |
| DYCP-32B | 130*200, 130*150 | 800 | 1~8 | 4~208 |
| DYCP-32C | 250*250, 120*250, 60*250 | 1600 | 1~4 | 26~204 |
| DYCP-44N | 100*200 | 2000 | 1~12 | 8~96 |
| DYCP-44P | 140*170 | 2000 | 1~6 | 17~198 |
For more information on agarose gel electrophoresis, feel free to contact us. Our professional lab technicians are available to assist you.
Beijing Liuyi Biotechnology Co., Ltd. (Liuyi Biotechnology) has over 50 years of expertise in manufacturing electrophoresis instruments. With a dedicated technical team and R&D center, we provide reliable and comprehensive production, from design to inspection and warehousing. We offer a full range of products, including:
- Electrophoresis Cells (Tanks/Chambers)
- Electrophoresis Power Supplies
- Blue LED Transilluminators
- UV Transilluminators
- Gel Imaging & Analysis Systems
In addition, we supply other laboratory instruments such as PCR machines, vortex mixers, and centrifuges.
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Post time: Nov-12-2024