Do you have any difficulties in preparing agarose gel? Let follow up with our lab technician in preparing the gel.
The preparation process of agarose gel involves the following steps:
Weighing Agarose Powder
Weigh the required amount of agarose powder according to the desired concentration for your experiment. Common agarose concentrations range from 0.5% to 3%. Higher concentrations are used for separating smaller DNA molecules, while lower concentrations are for larger molecules.
Preparing Buffer Solution
Add the agarose powder to an appropriate electrophoresis buffer, such as 1× TAE or 1× TBE. The volume of buffer should match the volume of gel needed for your experiment.
Dissolving the Agarose
Heat the agarose and buffer mixture until the agarose completely dissolves. This can be done using a microwave or a hot plate. Stir the solution intermittently to prevent it from boiling over. The agarose solution should become clear without any visible particles.
Cooling the Agarose Solution
Allow the heated agarose solution to cool to around 50-60°C. Stir the solution during the cooling process to prevent premature solidification.
Adding Nucleic Acid Stain (Optional)
If you want to visualize DNA or RNA in the gel, you can add a nucleic acid stain, such as GelRed or ethidium bromide, at this stage. When handling these stains, wear gloves and exercise caution, as they can be toxic.
Casting the Gel
Pour the cooled agarose solution into a prepared electrophoresis gel mold. Insert a comb to create sample wells, ensuring the comb is secure and the solution is evenly distributed in the mold.
Gel Solidification
Allow the gel to solidify at room temperature, which typically takes 20-30 minutes depending on the concentration and thickness of the gel.
Removing the Comb
Once the gel has fully solidified, carefully remove the comb to reveal the sample wells. Place the gel along with the mold into the electrophoresis chamber and cover it with the appropriate amount of electrophoresis buffer, ensuring the gel is fully submerged.
Preparing for Electrophoresis
After the gel is ready, load your samples into the wells, and proceed with the electrophoresis experiment.
If you have any issues with gel preparation, feel free to contact us. We have professional lab technicians available to communicate with you.
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Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) has specialized in manufacturing electrophoresis instruments for more than 50 years with our own professional technical team and R&D center. We have reliable and complete production line from design to inspection, and warehouse, as well as marketing support. Our main products are Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc. We also supply the lab instruments like PCR instrument, vortex mixer and centrifuge for laboratory.
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Post time: Oct-15-2024