A Brief Overview of Agarose Gel Electrophoresis
Agarose gel electrophoresis is a widely used technique in molecular biology for the separation of nucleic acids, such as DNA and RNA, based on their size. This method employs a gel made from agarose, a natural polysaccharide derived from seaweed. The gel acts as a molecular sieve, allowing the molecules to migrate through it when an electric current is applied. This separation process helps researchers analyze and characterize nucleic acid samples by observing the distinct bands formed according to their sizes. Agarose gel electrophoresis is a fundamental tool for tasks like DNA fragment analysis, purity assessment, and molecular biology research.
Normally, the agarose gel electrophoresis tank is called horizontal electrophoresis tank as well. The major applications of agarose gel electrophoresis include:
DNA Fragment Analysis:
Separation and analysis of DNA fragments based on size, essential for tasks such as genotyping and DNA fingerprinting.
RNA Analysis:
Resolution of RNA molecules, aiding in the study of gene expression patterns and RNA integrity.
PCR Product Confirmation:
Verification of polymerase chain reaction (PCR) products to ensure successful amplification and obtain accurate fragment sizes.
Plasmid DNA Analysis:
Examination of plasmid DNA for quality control, verification of cloning experiments, and identification of recombinant DNA.
Purity Assessment:
Determination of the purity of nucleic acid samples by visualizing potential contaminants or impurities.
Molecular Biology Research:
Fundamental tool in molecular biology laboratories for a variety of experiments, including DNA sequencing preparations and restriction enzyme digests.
Beijing Liuyi Biotechnology Co., Ltd. (formerly Beijing Liuyi Instrument Factory) was founded in 1970. It is a technology enterprise specializing in the production of biochemistry and molecular biology laboratory analytical instruments.Liuyi has specialized in manufacturing electrophoresis instruments for more than 50 years, and it has reliable and complete production line from design to inspection, and warehouse, as well as marketing support. The main products are Electrophoresis Cell (tank/chamber), Electrophoresis Power Supply, Blue LED Transilluminator, UV Transilluminator, Gel Image & Analysis System etc.
Let us know more about its one model of agarose gel electrophoresis-DYCP-31DN.
Introduction of agarose gel electrophoresis tank DYCP-31DN
Dimension (LxWxH) |
310×150×120mm |
Gel Size (LxW) |
60×60mm, 60×120mm, 120×60mm, 120×120mm |
Comb |
2+3 wells, 6+3 wells, 8+18 wells, and 11+25 wells |
Comb Thickness |
1.0mm, 1.5mm and 2.0mm |
Number of Samples |
2-100 |
Buffer Volume |
650ml |
Weight |
1.0kg |
DYCP-31DN is of Liuyi’s agarose gel electrophoresis tank, with a casting device available for casting 1-4 pieces gels. It can make two small size gel as size 60x60mm and one wide gel 120x60mm or a long gel 60x120mm at same time. The biggest gel that it can cast is size 120x120cm.
The comb thickness offered are 1.0mm, 1.5mm and 2.0mm. With the design of removable electrode, it is very easy to clean and maintain. “Bridge design” aimed at conserving buffer solution during the electrophoresis process. Efficient buffer usage is crucial for maintaining stable electric fields and ensuring accurate separation of molecules.
Other key futures:
1) The lids and the main tank bodies (buffer tanks) are transparent, molded, exquisite, durable, good seal, no chemical pollution;chemical-resistant, pressure-resistant.
2) Electrodes are made by pure platinum(the purity quotient of the noble metal ≥99.95%) which have the features of corrosion resistance of electroanalysis and withstand high temperature, the function of electric conduction is very good.
3) Sample wells are often difficult to see, so the black band on the gel tray makes it convenient to observe and to load the samples.
4) Power fails when you open the lid for the user’s safe.
There are several models of power supply offered for DYCP-31DN. The hottest electrophoresis power supply is DYY-6C. Welcome to visit our website for more models of power supply.
For visualizing the agarose gel with DNA or RNA , Liuyi provides UV transilluminators for the customers. WD-9403C, WD-9403B and WD-9403X are all available for customer to choose.
Agarose gel electrophoresis is useful for tasks such as checking the size of DNA fragments, assessing the purity of a DNA sample, or separating different DNA fragments for further analysis.
If you want to know more details of this product, please don’t hesitate to contact us. Visiting us by www.gelepchina.com, or you can send us message at email [email protected] or [email protected], or please call us at +86 15810650221 or add Whatsapp +86 15810650221, or Wechat: 15810650221.
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Post time: Jan-11-2024