Polyacrylamide is often used in molecular biology applications as a medium for electrophoresis of proteins and nucleic acids in a technique known as PAGE. It is a kind of zone electrophoresis method by synthetics gel called polyacrylamide as a supporting medium. It was built by S.Raymond and L.Weintraub in 1959, and then promoted and developed by L.Ornstein and B.J. Davis. This method was widely used after further explanation and modification by them in theory and experimental technique in 1964.
Before applying polyacrylamide gel electrophoresis, people mainly use paper electrophoresis for zone EP. But the paper act as a medium, just have the function of anti-convection, no any other positive effect. While the polyacrylamide gel not only has the function of anti-convection but also it can be participated in the separation proceeding actively. Because polyacrylamide gel is a kind of net structure, which is a polymerization and cross-linking combination of acrylamide (Acr) and N,N-methylenebis(acrylamide). Acrylamide is called monomer, while the N,N-methylenebis is called a comonomer or crosslinker. The forming of a gel is a process of chemical polymerization. The pore size of a gel can be controlled, thus the gel can be made with different cross-linking degrees. If the pore size is approaching the average radius of the sample’s molecule, the resistance of the molecule to go through the gel pore will have a close relationship with the size and shape of the molecule during the electrophoresis. So it provides a changeable separation factor for separating those materials with similar net charges.
There are two general ways used for polyacrylamide gel electrophoresis, one is disc electrophoresis, and the other one is slab electrophoresis. The slab electrophoresis is specially designed for separating protein and DNA, and generally, there are two kinds of slab electrohporesis, which are the horizontal electrophoresis tank and the vertical electrophoresis tank. For the protein, people use the horizontal electrophoresis tank for IFF and immunoelectrophoresis, otherwise, people use the vertical electrophoresis tanks for proteins.
Beijing Liuyi Biotechnology has various kinds of slab electrophoresis tanks for PAGE, except for analysis and identification of protein samples by polyacrylamide gel electrophoresis, it also can be used for measuring samples’ molecular weight, purifying samples and preparing samples.
Take the model DYCZ-23A as an example, which is a typical vertical electrophoresis tank for Lab. Use two glass plates to form a gel room to make a gel, and then clap the glass plates tightly to prevent gel leakage. The thickness of the gel is depended on the spacer’s thickness. Normally, the thickness is 1.5mm for better heat releasing, and DYCZ-23A also provides 1.0mm thickness spacers for casting 1.0mm gel. Except electrophoresis tank, in order to run electrophoresis, the power supply is also needed. Beijing Liuyi Biotechnology offers a variety of electrophoresis power supply. From high voltage to lower voltage, you choose the model according to the application.
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Post time: Oct-12-2022