Here we will describe how to perform agarose gel electrophoresis by our researcher in Liuyi Biotech’s lab.
Before the experiment, we need to check the apparatus, reagents, and other experimental materials and tools we need.
Preparation of experimental apparatuses and materials
The apparatuses for agarose gel electrophoresis
Horizontal electrophoresis cell(tank/chamber), middle and lower voltage electrophoresis power supply, gel image & analysis system.
Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) offers different models of horizontal electrophoresis cell (tank/chamber) and power supply as well as the gel documentation system. The model DYCP-31 series products are for electrophoresis, and DYY series products are the electrophoresis power supply. The WD-9413 series products are the gel documentation system. From small gel size 60×60mm to massive gel 250×250mm, we can meet your different requirements for gel size. The model DYCP-32C can make the gel to reach size 250×250mm. The DYY-6C is our power supply. It supports an output of 400V, 400mA, 240W, which is our common product used by our customers. WD-9413B Gel Image & Analysis System is used for analyzing and researching for the gel, films, and blots after the electrophoresis experiment. It is a basic device with ultraviolet light source for visualizing and photographing the gels stained with fluorescent dyes like ethidium bromide, and with white light source for visualizing and photographing the gels stained with dyes like coomassie brilliant blue.
The reagents for agarose gel electrophoresis
1.The medium: agarose gel
2.The buffer: TAE (Tris-acetate, EDTA, glacial acetic acid) and TBE ( Tris-borate, EDTA, boric acid ).
3.The loading buffer: 6×DNA Loading Buffer (Special for DNA sample: EDAT, glycerine,Xylene cyanol and bromophenol blue)
The Dye
The fluorescent dye like EB, Gelred, goldview, GenGreen, GenView, SYBRGreen
The Consumables
Sterilization pipette tips (10μL), pipette tips (200μL), pipette tips (1000μL), 200μL\500μL\1.5ml centrifuge tube.
DNA Marker
Prepared according to the molecular weight to be tested.
The Gel running and observe
First, we need to prepare the agarose gel. The concentration of agarose in a gel will depend on the sizes of the DNA fragments to be separated, with most gels ranging between 0.5%-2%. Taking our DYCP-31DN for example, You can cast small gel, wide gel , long gel and square gel according to the requirements of the experiment. Chose the gel tray you need, and insert the comb, then Pour the heated agarose gel into the gel casting device.
Then, after the gel becomes solid, take the comb away carefully and softly, and then put the gel tray into the buffer tank. Pour the buffer solution into the buffer tank and make all the gel immersed in the buffer. Load the samples into the wells with a standard pipette. Connect the DYCP-31DN with the electrophoresis power supply correctly, and set the parameters to run the gel.
Visualize your DNA fragments in a UV transilluminator.
After running the gel, you can use our gel image & analysis system model WD-9413B to observe, analyze and take pictures for the gel. Liuyi Biotech also offers UV transilluminator (UV Analyzer) to observe the gel. We have black-box type UV transilluminator (UV Analyzer) model WD-9403A, 9403C, WD-9403F, portable UV transilluminator (UV Analyzer) model WD-9403B and handhold UV transilluminator (UV Analyzer) WD-9403E for you choose.
An image of a gel post electrophoresis
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Post time: Apr-29-2022