DYCZ-40D Electrode Assembly

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Cat.No.: 121-4041

The electrode assembly is matched with DYCZ-24DN or DYCZ-40D tank. Used to transfer the protein molecule from the gel to membrane like nitrocellulose membrane in the Western Blot experiment.

Electrode assembly is the important part of DYCZ-40D,which has the capacity to hold two gel holder cassettes for electrophoresis transfer between the parallel electrodes only 4.5 cm apart. The driving force for blotting applications is the voltage applied over the distance between the electrodes. This short 4.5 cm electrode distance allows generation of higher driving forces to produce efficient protein transfers. Other features of DYCZ-40D includes latches on the gel holder cassettes for easy handling purpose, supporting body for transfer (electrode assembly) comprise of red and black color parts and red and black electrodes to ensure proper orientation of the gel during the transfer, and an efficient design which simplifies insertion and removal of the gel holder cassettes from the supporting body for transfer (electrode assembly).

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An electrophoresis system consists of two main components: a Power Supply and an Electrophoresis Chamber.The power supply supplies power. The "power," in this case, is electricity. The electricity that comes from the power supply flows, in one direction, from one end of the electrophoresis chamber to the other. The cathode and anode of the chamber are what attracts oppositely charged particles.

Inside the electrophoresis chamber, is a tray--more precisely, a casting tray. The casting tray consists of the following parts: glass plate which goes in the bottom of the casting tray. The gel is held in the casting tray. The "comb" looks like its name.The comb is placed in slots on the side of the casting tray.It is put in the slots BEFORE the hot, melted gel is poured. After the gel solidifies, the comb is taken out. The "teeth" of the comb leave small holes in the gel that we call "wells." Wells are made when the hot, melted gel solidifies around the teeth of the comb. The comb is pulled out after the gel has cooled, leaving wells. The wells provide a place to put the particles you wish to test. A person must be very careful not to disrupt the gel when loading the particles. Cracking, or breaking the gel will likely affect your results.